The best Side of cytometer
The best Side of cytometer
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Info-enriched characterization of mobile populations. Morphological information and facts from illustrations or photos can increase for the richness of flow cytometry facts. Such as, the figure demonstrates an usually standard apoptosis assay applying Annexin V and PI, adding mobile imaging to characterize cells in each population to expose morphologically distinct functions. These insights couldn't happen to be received from multiplex staining on your own.
As samples are acquired over the Attune CytPix Flow Cytometer, the significant-pace brightfield camera captures and shops photos of detected activities at a rate as much as six,000 visuals for each 2nd, depending upon the flow rate and impression size. For increased adaptability, Attune Cytometric Program permits you to modify the image capture frequency as desired and choose a selected gate for impression seize, if expected.
(B) RAW 264.7 macrophages tend to be annotated inaccurately with regard to particle depend because of their complexity and noncircular borders. The volume of singlets obtainable for Investigation increases as soon as the consumer trains and employs a far more correct design.
Find out new insights into your experimental knowledge by visualizing populations applying put together fluorescence and morphology facts. Our software package interface wizards permit downsampling, concatenation (merged files) and dimensionality applications which might be created in to the program for an intuitive, streamlined data Evaluation working experience.
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Additionally, it is actually illuminating to discover the number of doublets slip as a result of traditional singlet gates, and excellent to verify the position of surprising double-positive activities in my Investigation."
Optimizing gating technique. Even strong guide singlet gating is error-susceptible and remains a subjective determination stage in Virtually all flow cytometry assays. Imaging can be used to substantiate and modify gates to incorporate only single cells of desire.
Our researchers will structure panels for you as repeatedly you'll need—totally free, no merchandise acquire necessary.
The Attune NxT cytometer is the dependable workhorse instrument your lab can depend upon for consistent and trustworthy effectiveness. The Attune CytPix design includes a superior-velocity brightfield digicam and AI-pushed graphic Evaluation software, with recent upgrades which include equipment Discovering equipment and an intuitive consumer interface, enabling enhanced details precision, precision, and more quickly insights into sample biology.
Possibly most significantly, these functions include cells of Obviously diverse phenotypes which could lead on to incorrect conclusions pertaining to double good situations (especially in unusual populations).
Seem waves enable precise mobile alignment, causing Remarkable facts high quality and ~10X more rapidly sample flow premiums.
Software Observe: Automated picture Assessment reduces user-to-user variability in flow cytometry gating methods
Leverage the power of Highly developed technological know-how and innovation to accelerate your discoveries. Believe in within the Attune Flow Cytometer family to provide the trustworthiness, pace, and accuracy that established your get the job done aside.
Adaptable payment approach from Agilent Money Alternatives allow you to obtain the critical instrumentation you require for rapid project, or to grow your lab.
Gating on these extended parameters means that you can immediately and accurately discover populations of desire and ensure gating strategy with little or no guide evaluation. The Attune software package features use of condition-of-the-art machine Discovering analysis instruments in an expertly intended user interface, making it exceptionally simple to use.
The Attune Xenith cytometer is really a large-parameter spectral analyzer, furnishing both of those spectral unmixing and conventional flow abilities for in depth and multipurpose mobile Examination.
The Attune CytPix Flow Cytometer permits customers to visualise samples in get more info the course of acquisition, validate the accuracy of gating techniques, and support success with reliable documentation for publication applications.
Here, we display that by gating techniques working with picture-primarily based quantitative parameters can distinguish interacting cells from coincident gatherings to more accurately examine interacting cells.